Datasheet
SDS
Description
Introduction
| The AmMag Quatro is an automated solution for purification of large-scale, high quality, transfection-grade plasmids from Escherichia coli cultures. The solution consists of a system controller that provides simultaneous operation of up to 4 independent purification modules, along with pre-filled consumables and verified protocols.
The consumable kit is an all-inclusive solution that contains the samples tube, reaction tube, reagent cartridge, tip box that contains the elution tube and the waste container. |
AmMag Quatro Product Components
| Product | Description | Catalog Number |
|---|---|---|
| AmMag™ Quatro System Controller | One controller can control up to 4 purification modules, simultaneously | D00018 |
| AmMag™ Quatro Automation Purification Module | Purify up to 6 samples in one run | D00019 |
| AmMag™ Quatro Plasmid Purification TE Kit | Elution buffer: Tris EDTA – TE buffer 24 samples |
L00943-24 |
| AmMag™ Quatro Plasmid Purification H<sub>2</sub>O Kit | Elution buffer: Water – H2O 24 samples |
L00882-24 |
Overview
The AmMag Quatro leverages magnetic bead technology, and alkaline lysis related reagents to automate maxi-scale plasmid purification in three easy steps.
Step 1. The plasmid purification process starts with the centrifuged bacterial pellet.
Step 2. The automated steps consist of pellet resuspension, lysis, neutralization,pre-clarification, plasmid DNA binding, endotoxin removal, washes, and elution.
Step 3. The optional manual centrifugation step of the purified plasmid DNA removes residual endotoxins, trace amounts of beads, and sterile filters the final purified plasmid DNA.
The AmMag Quatro system consists of the purification module(s) and main controller.
The main controller provides direct control over the operation of the purification modules.
Up to 4 purification modules can be connected via a network cable, and controlled independently.
| Degree of automation | Full – starting with pelleted bacterial culture and ending with transfection grade pure plasmid |
| Sample type | Escherichia coli - E. coli |
| Sample throughput | 6 samples per purification module
24 samples with a 4 purification module setup |
| Plasmid sample size | Bacteria wet pellet mass ≤ 1.5 g |
| Estimated run time | ~2 hours – depending on purification program |
| Sample/Reaction Tube | AmMag Quatro 50 mL Tubes |
| Reagent storage conditions | Room Temperature – RT |
| Purification method | Magnetic beads method based on alkaline lysis |
| Dimensions Width x Depth x Height (L x W x H) |
System Controller : 382 mm X 416 mm X 249 mm Purification Module: 460 mm X 721 mm X 797 mm |
| Installation environment | Ambient temperature: 18 ℃ ~ 32 ℃ Relative humidity: ≤ 85% Altitude: ≤ 2000 m NOTE: avoid exposure to sources of electromagnetic interference |
| Power supply | Voltage: 100-240 V AC Frequency: 50/60 Hz Power Rating: Controller: 100 W Automation Purification Module: 500 W |
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